期刊
JOURNAL OF BIOTECHNOLOGY
卷 336, 期 -, 页码 1-9出版社
ELSEVIER
DOI: 10.1016/j.jbiotec.2021.06.015
关键词
Antifungal; Mushroom; Agaricus bisporus; Antimicrobial; Cyclic lipopeptide
资金
- Free State of Thuringia [2019 FGR 0079]
- International Leibniz Research School for Microbial and Biomolecular Interactions (ILRS)
- Infect Control [03ZZ0835A, 03ZZ0826A]
The research has found that jagaricin is a promising antifungal agent for agricultural applications, with high antifungal activity against human pathogenic fungi and almost non-toxic to plants.
In today's, society multi-resistant pathogens have become an emerging threat, which makes the search for novel anti-infectives more urgent than ever. A promising class of substances are cyclic lipopeptides like the antifungal jagaricin. Jagaricin is formed by the bacterial mushroom pathogen Janthinobacterium agaricidamnosum. It has shown antifungal activity against human pathogenic fungi like Candida albicans and Aspergillus fumigatus. In addition, jagaricin is nearly non-toxic for plants, which makes it a promising agent for agricultural applications. Cyclic lipopeptides formed by microorganisms originate from their secondary metabolism. This makes it very challenging to determine the inducing factor for product formation, especially for unknown microbial systems like J. agaricidamnosum. In the presented study, a biotechnological process for jagaricin formation was developed, investigating impact factors like the medium, oxygen availability, and phosphate. For this reason, experiments were conducted on microtiter plate, shake flask, and stirred tank bioreactor level. Ultimately, a final maximum jagaricin concentration of 251 mg L-1 (15.5 mg(Jagaricin)center dot g(CDW)(-1)) could be achieved, which is an increase of approximately 458 % in comparison to previous results in standard glucose medium. This concentration allows the production of significantly higher amounts of jagaricin and enables further experiments to investigate the potential of this substance.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据