期刊
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
卷 40, 期 22, 页码 12404-12412出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/07391102.2021.1971560
关键词
Bovine serum albumin; cuminol; fluorescence; molecular dynamics; molecular docking
资金
- Deanship of Scientific Research, King Saud University
The study showed weak binding between cuminol and BSA, mainly through a static quenching mechanism. The binding was supported by hydrophobic forces with a minor contribution from hydrogen bonding. While the secondary structure of BSA remained relatively unchanged at low cuminol concentrations, partial unfolding occurred with higher cuminol concentrations.
Cuminol (4-Isopropylbenzyl alcohol), found in the essential oils of several plant sources, is an important constituent of several cosmetics formulations. The interaction of cuminol with model plasma protein bovine serum albumin was studied in this paper. The experimental studies were mainly carried out using fluorescence spectrophotometry aided with UV visible and CD spectroscopies. Intrinsic fluorescence measurements showed that there was a weak binding between cuminol and BSA. The mechanism of binding involved static quenching with around 1:1 binding. The binding was chiefly supported by hydrophobic forces although a little contribution of hydrogen bonding was also found in the interaction and the values of enthalpy change were negative with positive entropy change. The secondary structure of BSA didn't change significantly in presence of low concentrations of cuminol, however, partial unfolding of the former taken place when the concentration of the latter increased. Molecular docking analyses showed cuminol binds at the intersection of subdomains IIA and IIIA, i.e. its binding site is in between Sudlow sites I and II. Molecular dynamics simulations results have shown that BSA forms a stable complex with cuminol and the structure of the former didn't change much in presence of later.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据