期刊
JOURNAL OF BASIC MICROBIOLOGY
卷 61, 期 8, 页码 709-720出版社
WILEY
DOI: 10.1002/jobm.202100221
关键词
in silico; Nigrospora sphaerica; quercetin; toxicity; trypsin inhibition
类别
The study identifies N. sphaerica as capable of producing the bioactive compound quercetin, which is considered a potential candidate for trypsin inhibition. Out of 10 endophytic fungi isolated, only one strain with the required activity was screened, with the active fungus identified as Nigrospora sphaerica obtained from Dendrophthoe falcata.
Trypsin is a protein-digesting enzyme that is essential for the growth and regeneration of bone, muscle, cartilage, skin, and blood. The trypsin inhibitors have various role in diseases such as inflammation, Alzheimer's disease, pancreatitis, rheumatoid arthritis, cancer prognosis, metastasis and so forth. From 10 endophytic fungi isolated, we were able to screen only one strain with the required activity. The fungus with activity was obtained as an endophyte from Dendrophthoe falcata and was later identified as Nigrospora sphaerica. The activity was checked by enzyme assays using trypsin. The fungus was fermented and the metabolites were extracted and further purified by bioassay-guided chromatographic methods and the compound isolated was identified using gas chromatography-mass spectrometry. The compound was identified as quercetin. Docking studies were employed to study the interaction. The absorption, distribution, metabolism, and excretion analysis showed satisfactory results and the compound has no AMES and hepatotoxicity. This study reveals the ability of N. sphaerica to produce bioactive compound quercetin has been identified as a potential candidate for trypsin inhibition. The present communication describes the first report claiming that N. sphaerica strain AVA-1 can produce quercetin and it can be considered as a sustainable source of trypsin active-site inhibitors.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据