β-estradiol promotes the growth of primary human fetal spermatogonial stem cells via the induction of stem cell factor in Sertoli cells

Background Mammalian spermatogenesis is responsible for male fertility and is supported by the self-renewal and differentiation of spermatogonial stem cells (SSCs). Sertoli cells provide a supportive microenvironment for SSCs, in part by the production of stem cell factor (SCF), which is a potent regulator of spermatogonia proliferation and survival. Methods We investigated the novel role of beta-estradiol in modulating the proliferation and apoptosis of fetal SSCs via the regulation of SCF secretion in Sertoli cells isolated from human fetal testes. The proliferation of SSCs in the co-culture system was determined by colony formation and BrdU incorporation assays. TUNEL assay was used to measure SSC apoptosis in co-culture in response to treatment with control, beta-estradiol, or the combination of beta-estradiol and the estrogen receptor inhibitor ICI 182780. Results In the system with purified human fetal Sertoli cells (MIS+/c-Kit-/AP-), beta-estradiol upregulated the production of SCF in a dose- and time-dependent manner. In the co-culture system of primary human fetal SSCs (c-Kit+/SSEA-4+/Oct-4+/AP+) and Sertoli cells (MIS+), beta-estradiol markedly increased the proliferation of SSCs. Moreover, SSC apoptosis was significantly inhibited by beta-estradiol and was completely reversed by the combination of beta-estradiol and ICI 182780. Conclusion Here we report, for the first time, that beta-estradiol can induce the increase of SCF expression in human fetal Sertoli cells and regulates the growth and survival of human fetal SSCs. These novel findings provide new perspectives on the current understanding of the role of estrogen in human spermatogenesis.

Automated summary

Mammalian spermatogenesis relies on the self-renewal and differentiation of spermatogonial stem cells (SSCs) with support from Sertoli cells. This study revealed that beta-estradiol can regulate the proliferation and survival of fetal SSCs by modulating the secretion of stem cell factor (SCF) in Sertoli cells, offering new insights into the role of estrogen in human spermatogenesis.