期刊
FOOD CHEMISTRY
卷 200, 期 -, 页码 154-158出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2016.01.033
关键词
Stevia leaves; Transglycosylation; Cellulase; Glucosyl donors; Rebaudioside-A
资金
- Council of Scientific and Industrial Research (CSIR), New Delhi [CSC-0133]
- CSIR
Rebaudioside-A is the second most abundant sweet diterpene glycoside (1-3%) present in the leaves of Stevia rebaudiana Bertoni, and is now being considered as a possible sucrose substitute due to its pleasant organoleptic properties and associated health benefits. In the present study, a novel in situ enzymatic transglycosylation of stevioside has been developed by pre-treating the stevia leaves with cellulase and adding soluble starch as the glucosyl donor. The results confirm that the transglycosylation of stevioside led to an enrichment in the rebaudioside-A content from 4% to 66%. This was further purified by multiple column chromatography to obtain 95% pure rebaudioside-A. The isolated rebaudioside-A showed concentration-dependent alpha-glucosidase inhibitory activity with IC50 = 35.01 mu g/ml. Thus the study highlights the biotransformation of stevioside present in stevia leaves to rebaudioside-A by a simple, inexpensive and eco-friendly process that has commercial potential. (C) 2016 Elsevier Ltd. All rights reserved.
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