期刊
FOOD CHEMISTRY
卷 199, 期 -, 页码 760-767出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2015.12.070
关键词
BODIPY665/676; Radicals; Two-photon irradiation; Lipid oxidation; Oil droplet; CLSM
资金
- Danish Agency of Science, Technology and Innovation
The initiation of lipid autoxidation within single oil droplets in Tween-20-stabilized oil-in-water emulsion was achieved by highly focused two-photon (2P) irradiation at excitation wavelength (lambda(ex)) 700 nm. The radical formation was enhanced by inclusion of the photo-cleavable radical initiator di-tert-butyl peroxide (DTBP) into the droplets, and demonstrated with confocal microscopy using radical-sensitive probe BODIPY665/676. The radical chain reactions progressed up to 60 mu m; however, there were no indications of oxidation in neighboring droplets demonstrating that radicals and oxidized probe molecules were not able to migrate between oil droplets. In addition, the spatial propagation of lipid autoxidation increased with the degree of oil unsaturation. (C) 2015 Elsevier Ltd. All rights reserved.
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