期刊
JOURNAL OF AQUATIC FOOD PRODUCT TECHNOLOGY
卷 30, 期 7, 页码 907-913出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/10498850.2021.1948478
关键词
Hdca gene; alkaline-fermented fish; real-time pcr; food safety
This study quantified histamine content in Hakarl for the first time and detected the hdcA gene of Gram-positive bacteria, suggesting that the low histamine levels in the samples may be associated with the decarboxylase activity of Gram-negative bacteria. Further research on histamine levels and microbiota dynamics during production processes is needed.
Hakarl is a fermented fish traditionally produced in Iceland. In the present study, no scientific research has so far discussed the implication of histamine in this food. The first quantification of histamine in ready-to-eat samples of hakarl has been performed. The presence of the hdcA gene of Gram-positive bacteria, directly detected in the food matrix via qPCR, was also assessed. Histamine content was between 8.3 +/- 0.8 and 13.2 +/- 0.3 mg/Kg, with an overall mean of 10.6 +/- 2.0 mg/Kg. Interestingly, the copy number of the hdcA gene carried by Gram-positive bacteria harbored by hakarl was below the detection limit in all the samples. Therefore, although the involvement of such a bacterial group cannot be excluded, the results suggest that the low histamine levels detected in the samples can presumably be associated with the decarboxylase activity of Gram-negative bacteria. The present study represents a step forward in understanding the microbiology of hakarl, although further investigations dealing with the monitoring of both histamine levels and microbiota dynamics during the production process are needed.
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