4.5 Article

Carnosine, oxidative and carbonyl stress, antioxidants, and muscle fiber characteristics of quadriceps muscle of patients with COPD

期刊

JOURNAL OF APPLIED PHYSIOLOGY
卷 131, 期 4, 页码 1230-1240

出版社

AMER PHYSIOLOGICAL SOC
DOI: 10.1152/japplphysiol.00200.2021

关键词

carbonyl stress; carnosine; chronic obstructive pulmonary disease; oxidative stress; quadriceps

资金

  1. Flemish Government
  2. FWO [11B4718N]
  3. Limburgs Kankerfonds

向作者/读者索取更多资源

Carnosine, a skeletal muscle antioxidant mainly found in fast-twitch fibers, was studied in the quadriceps of COPD patients. Despite similar levels in COPD patients and healthy controls, carnosine was 31% lower in severe/very severe COPD patients compared to mild/moderate cases, suggesting a potential early indicator of muscle redox balance abnormalities.
Oxidative/carbonyl stress is elevated in lower-limb muscles of patients with chronic obstructive pulmonary disease (COPD). Carnosine is a skeletal muscle antioxidant particularly present in fast-twitch fibers. The aims of the present study were to compare muscle carnosine, oxidative/carbonyl stress, antioxidants, and fiber characteristics between patients with COPD and healthy controls (HCs) and between patients after stratification for airflow limitation (mild/moderate vs. severe/very severe), as well as to investigate correlates of carnosine in patients with COPD. A vastus lateralis muscle biopsy was obtained from 40 patients with stable COPD and 20 age- and sex-matched HCs. Carnosine, oxidative/carbonyl stress, antioxidants, fiber characteristics, quadriceps strength and endurance (QE), V(O2)peak (incremental cycle test), and physical activity (PA) were determined. Patients with COPD had a similar carnosine concentration [4.16mmol/kg wet weight (WW; SD = 1.93)] to HCs [4.64mmol/kg WW (SD = 1.71)] and significantly higher percentage of fast-twitch fibers and lower QE, V(O2)peak, and PA versus HCs. Patients with severe/very severe COPD had a 31% lower carnosine concentration [3.24mmol/kg WW (SD = 1.79); n = 15] versus patients with mild/moderate COPD [4.71mmol/kg WW (SD = 1.83); n = 25; P = 0.02] and significantly lower V(O2)peak and PA versus patients with mild/moderate COPD. Carnosine correlated significantly with QE (r(s) = 0.427), V(O2)peak (r(s) = 0.334), PA (r(s) = 0.379), and lung function parameters in patients with COPD. In conclusion, despite having the highest proportion of fast-twitch fibers, patients with severe/very severe COPD displayed a 31% lower muscle carnosine concentration compared with patients with mild/moderate COPD. As no other markers of oxidative/carbonyl stress or antioxidants were affected, the observed carnosine deficiency is thought to be a possible first sign of muscle redox balance abnormalities. NEW & NOTEWORTHY Carnosine, particularly present in fast-twitch fibers, was investigated in the quadriceps of patients with chronic obstructive pulmonary disease (COPD). Carnosine concentration was similar between patients with COPD and healthy controls but was 31% lower in patients with severe/very severe COPD, despite their high proportion of fast-twitch fibers, versus patients with mild/moderate COPD. As no other markers of oxidative/carbonyl stress or antioxidants were affected, the observed carnosine deficiency is thought to be a possible first sign of muscle redox balance abnormalities.

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