4.6 Article

Isolation and identification of a pathogenic bacterium, Exiguobacterium oxidotolerans XP-2, from the abnormal diseased mature sporophytes of a commercially cultivated brown seaweed Saccharina japonica

期刊

JOURNAL OF APPLIED PHYCOLOGY
卷 33, 期 5, 页码 3239-3249

出版社

SPRINGER
DOI: 10.1007/s10811-021-02499-9

关键词

Abnormal disease; Exiguobacterium oxidotolerans XP-2; Green-rotten disease; Pathogenic bacteria; Saccharina japonica

资金

  1. Marine S & T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao) [2018SDKJ0406-5]
  2. National Natural Science Foundation of China [41576158, 42076106]
  3. Fundamental Research Funds for the Central Universities [201822025]
  4. National Key R&D Program of China [2018YFD0900305]
  5. Rongcheng Marine Industrial Technology Research Institute, Ludong University [KF20180002]

向作者/读者索取更多资源

A Gram-positive bacterium XP-2 was isolated from diseased Saccharina japonica, causing green-rotten disease in healthy juvenile sporophytes. Analysis showed that XP-2 was closely related to Exiguobacterium oxidotolerans 12280(T), and its pathogenicity was identified by Koch's Postulate under laboratory conditions. This study enriched the list of pathogenic bacteria in commercially cultivated seaweeds and laid a foundation for further research on the virulence mechanisms of these pathogens.
Diseases of the commercially cultivated brown seaweed Saccharina japonica have been noticed since the 1960s in China. However, the usually opportunistic nature of the pathogenic bacteria of seaweeds is still a main obstacle for isolating and identifying pathogenic bacteria responsible for causing diseases of the cultivated S. japonica. In this study, a Gram-positive bacterium XP-2 was isolated from the abnormal diseased mature sporophytes of S. japonica. Pathogenicity test indicated that XP-2 could cause green-rotten disease in healthy juvenile sporophytes and significant algal ultrastructural changes were observed after S. japonica was infected by XP-2. The cells shrank, and the thylakoids of the chloroplasts became blurred after 12 h of re-infection. At 24 h slight deformation was observed in the structure of the mitochondria, chloroplasts, and nucleus. There also were many vacuoles in the infected cells. After 31 h of re-infection, mitochondrial structure and thylakoids became more indistinct. SEM and TEM observations indicated that XP-2 was rod-shaped with no flagella. Analysis of the full length 16S rRNA gene sequence and physiological and chemical characteristics indicated that XP-2 was closely related to Exiguobacterium oxidotolerans 12280( T). Based on these results, XP-2 was designated Exiguobacterium oxidotolerans XP-2. The pathogenicity of E. oxidotolerans XP-2 was identified by Koch's Postulate under laboratory conditions. These results enrich the growing list of pathogenic bacteria in commercially cultivated seaweeds and also provide a foundation for investigating the virulence mechanisms of pathogenic bacteria of commercially cultivated seaweeds in the near future.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.6
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据