期刊
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
卷 149, 期 3, 页码 943-956出版社
MOSBY-ELSEVIER
DOI: 10.1016/j.jaci.2021.09.013
关键词
Bacterial lysate; microbial interventions; OM-85; asthma; allergic inflammation; airway compartment; intranasal route; innate immunity; adaptive immunity
资金
- OM Pharma SA
- BIO5 Institute
- National Institutes of Health [P01AI148104, R21AI144722]
- [T32 ES007091]
- [T32 HL007249]
This study demonstrates that direct administration of OM-85 to the airway compartment can protect against experimental allergic asthma by engaging multiple immune pathways. The efficacy of intranasal OM-85 administration may be due to its direct access to the airway mucosal networks controlling the initiation and development of allergic asthma.
Background: Microbial interventions against allergic asthma have robust epidemiologic underpinnings and the potential to recalibrate disease-inducing immune responses. Oral administration of OM-85, a standardized lysate of human airways bacteria, is widely used empirically to prevent respiratory infections and a clinical trial is testing its ability to prevent asthma in high-risk children. We previously showed that intranasal administration of microbial products from farm environments abrogates experimental allergic asthma. Objectives: We sought to investigate whether direct administration of OM-85 to the airway compartment protects against experimental allergic asthma; and to identify protective cellular and molecular mechanisms activated through this natural route. Methods: Different strains of mice sensitized and challenged with ovalbumin or Alternaria received OM-85 intranasally, and cardinal cellular and molecular asthma phenotypes were measured. Airway transfer experiments assessed whether OM-85-treated dendritic cells protect allergen-sensitized, OM-85-naive mice against asthma. Results: Airway OM-85 administration suppressed allergic asthma in all models acting on multiple innate and adaptive immune targets: the airway epithelium/IL-33/ILC2 axis, lung allergen-induced type 2 responses, and dendritic cells whose Myd88/Trif-dependent tolerogenic reprogramming was sufficient to transfer OM-85-induced asthma protection. Conclusions: We provide the first demonstration that administering a standardized bacterial lysate to the airway compartment protects from experimental allergic asthma by engaging multiple immune pathways. Because protection required a cumulative dose 27-to 46-fold lower than the one reportedly active through the oral route, the efficacy of intranasal OM-85 administration may reflect its direct access to the airway mucosal networks controlling the initiation and development of allergic asthma.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据