期刊
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
卷 149, 期 3, 页码 1031-1043出版社
MOSBY-ELSEVIER
DOI: 10.1016/j.jaci.2021.07.040
关键词
Der p 37; house dust mite allergy; recombinant allergen; allergy diagnosis
资金
- Austrian Science Fund (FWF) [DK W 1248-B30, F4602, F4605]
- Country of Lower Austria and the
- Government of the Russian Federation [14.W03.31.0024]
- Medical University of Vienna
Der p 37, a new Dp allergen recognized by a third of HDM-allergic patients, may serve as a surrogate marker for severe HDM sensitization and asthma.
Background: House dust mite (HDM) allergens are major elicitors of allergic reactions worldwide. Objective: Identification, characterization, and evaluation of diagnostic utility of a new important HDM allergen was performed. Methods: A cDNA coding for a new Dermatophagoides pteronyssinus (Dp) allergen, Der p 37, was isolated from a Dp expression library with allergic patients' IgE antibodies. Recombinant Der p 37 (rDer p 37) expressed in Escherichia coli was purified, then characterized by mass spectrometry, circular dichroism, and IgE reactivity by ImmunoCAP ISAC technology with sera from 111 clinically defined HDM-allergic patients. The allergenic activity of rDer p 37 was studied by basophil activation and CD4(+) T-cell responses by carboxyfluorescein diacetate succinimidyl ester dilution assays. Specific antibodies raised against rDer p 37 were used for the ultrastructural localization of Der p 37 in mites by immunogold transmission electron microscopy. Results: Der p 37, a 26 kDa allergen with homology to chitin binding proteins, is immunologically distinct from Der p 15, 18, and 23. It is located in the peritrophic membrane of fecal pellets. Der p 37 reacted with IgE antibodies from a third of HDM-allergic patients and induced specific basophil-and CD4+ T-cell activation. Der p 37 IgE-positive patients had significantly higher IgE levels to major HDM allergens, reacted with more HDM allergens, and had a higher risk (odds ratio 5 3.1) of asthma compared to Der p 37-negative patients. Conclusions: Der p 37, a new Dp allergen recognized by a third of HDM-allergic patients, may serve as a surrogate marker for severe HDM sensitization and asthma.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据