期刊
FOOD AND CHEMICAL TOXICOLOGY
卷 95, 期 -, 页码 103-109出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fct.2016.06.012
关键词
3-acetyl-deoxynivalenol (3ADON); 15-acetyl-deoxynivalenol (15ADON); Deoxynivalenol-3-beta-D-glucoside (DON3G); IPEC-J2; Flow cytometry; Apoptosis; Necrosis
资金
- Ghent University Special Research Fund grant [I/00105/01]
- Vienna Science and Technology Fund [WWTF LS12-021]
- Austrian Science Fund (FWF) [SFB F3708]
- Austrian Federal Ministry of Science, Research and Economy
- Austrian National Foundation of Research, Technology and Development
The gastrointestinal tract is the first target after ingestion of the mycotoxin deoxynivalenol (DON) via feed and food. Deoxynivalenol is known to affect the proliferation and viability of animal and human intestinal epithelial cells. In addition to DON, feed and food is often co-contaminated with modified forms of DON, such as 3-acetyldeoxynivalenol (3ADON), 15-acetyl-deoxynivalenol (15ADON) and deoxynivalenol-3-beta-D-glucoside (DON3G). The goal of this study was to determine the in vitro intrinsic cytotoxicity of these modified forms towards differentiated and proliferative porcine intestinal epithelial cells by means of flow cytometry. Cell death was assessed by dual staining with Annexin-V-fluorescein isothiocyanate (FITC) and propidium iodide (PI), which allows the discrimination of viable (FITC-/PI-), apoptotic (FITC+/PI-) and necrotic cells (FITC-F/PI+). Based on the data from the presented pilot in vitro study, it is concluded that cytotoxicity for proliferative cells can be ranked as follows: DON3G << 3ADON < DON approximate to 15ADON. (C) 2016 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据