期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 69, 期 38, 页码 11171-11184出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.1c01719
关键词
wheat; metabolomics; transcriptomics; coexpression networks; anthocyanins
资金
- National Key Project of Transgenic Biologic Varieties Breeding of China [2016ZX08009003-004]
- Key Research and Developmental Projects in Sichuan Province [2019YFN0024]
- Strategic Priority Research Program of Chinese Academy of Sciences [XDA24030402]
- Central Public-interest Scientific Institution Basal Research Fund [Y2021XK04]
This study characterized the dynamic changes in anthocyanin components and transcripts in the grain of purple wheat ZNM168 and identified key pigments accumulated during grain development, as well as core genes and critical structural genes in the anthocyanin biosynthesis pathway. The findings provide insights for exploiting genetic engineering targets to breed anthocyanin-enriched wheat.
Purple wheat is thought to have beneficial effects on humans owing to its high anthocyanin content. However, a systematic understanding of the anthocyanin biosynthesis process in developing wheat grain is lacking. Here, the dynamic changes in anthocyanin components and transcripts in the grain of purple wheat ZNM168 at five developmental stages (10, 15, 20, 25, and 30 DAF) were characterized. Compared with other anthocyanins, four components, cyanidin 3-O-rutinoside, cyanidin 3-O-glucoside, cyanidin 3,5-O-diglucoside, and malvidin 3-O-glucoside, were significantly accumulated with grain development. In particular, the considerable accumulation of cyanidin 3-O-rutinoside indicated that it was the pivotal pigment for the purple grain. Transcriptome analysis revealed that the nine differentially expressed genes related to anthocyanin biosynthesis belonged to the BZ1 group, the homologous enzyme encoded by the maize Bronze-1 locus, which may primarily serve to glucosylate anthocyanidins. By constructing a gene coexpression network based on weighted gene coexpression network analysis, the TaBZ1 UniGene (TraesCS1D02G019200) was predicted as a core gene in anthocyanin biosynthesis. In addition, correlation analysis between the metabolites and transcripts suggested that TraesCS2A01G527700 (TaCHS) and TraesCS6B01G006200 (TaANS) were considered critical structural genes in the anthocyanin biosynthesis pathway. This study provides insights to exploit genes pinpointed as genetic engineering targets, thereby breeding anthocyanin-enriched wheat.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据