期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 69, 期 30, 页码 8471-8481出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.1c03249
关键词
Botryosphaeria dothidea; (R)-(-)-mellein; apple; pathogenic substance
资金
- National Key Research and Development Program of China [2016YFD0201122]
- Agricultural Science and Technology Innovation Project of Shandong Academy of Agricultural Sciences [2017CXGC0214]
- China Agriculture Research System [CARS-27]
- Taishan Scholar Construction Project of Shandong Province
- Research Foundation for Advanced Talents of Qingdao Agricultural University [663/1114318]
Botryosphaeria dothidea is a pathogenic fungus causing apple ring rot in China. The study found that the pathogenic substance mellein exists in infected apple fruits and branches, with different concentrations, and there is a relation between disease spot expansion and mellein production kinetics in apple tissue.
Botryosphaeria dothidea is a pathogenic fungus that can cause apple ring rot, a destructive apple disease in China. There have been reports on its molecular pathogenesis, but the pathogenic substances still remain unknown. In the present study, instrument analysis including UPLC-high-resolution mass spectrometry (HRMS) and nuclear magnetic resonance showed that B. dothidea fermentation broth contained (R)-(-)-mellein, a well-known fungal enantiomer of mellein. For further confirmation, a UPLC-MS/MS method for the determination of mellein was developed and validated. By this method, mellein was found to also exist in B. dothidea-infected apple fruits and branches with concentration ranges of 0.14-0.94 and 5.88-80.29 mg/kg, respectively. The concentration in fruits reached a peak at 48 h after pathogen inoculation, while a sustained concentration increase was achieved within 11 days for branches. Simultaneously, it was evident that there was a relation between disease spot expansion and mellein production kinetics in apple tissue. Phytotoxic bioassay showed that mellein could cause discoloration and death of apple leaves and browning in stems. Therefore, we confirmed that mellein was one of the pathogenic substances of B. dothidea. The present study provided additional data for the research on the pathogenesis of this pathogen.
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