期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 69, 期 49, 页码 14742-14750出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.1c03953
关键词
tropomyosin; glycation modification; allergenicity; hypoallergen; allergy desensitization; immunotherapy
资金
- China Postdoctoral Science Foundation [2021M693026]
- Qingdao Postdoctoral Applied Research Project [862105040045]
- Research Funding of Ocean University of China [862001013187]
This study found that glycation modification of TM with saccharides of different molecular sizes can lead to the generation of hypoallergenic GTM, with TM glycated by maltose exhibiting lower allergenicity changes upon activating allergic reactions of mast cells and mouse models. Hypoallergenic GTM + Al(OH)(3) was also shown to be an effective candidate immunotherapy for desensitizing allergic reactions in patients allergic to shrimp food, providing preclinical data to support its use.
Shrimp is a major allergic food that could trigger severe food allergy, with the most significant and potent allergen of shrimp referred to as tropomyosin (TM). Glycation modification (Maillard reaction) could reportedly weaken the allergenicity of TM and generate hypoallergenic TM, while up to now, there is still a lack of investigations on the hypoallergenic glycated tropomyosin (GTM) as a candidate immunotherapy for desensitizing the shrimp TM-induced allergy. This study analyzed the effects of glycation modification on decreasing the allergenicity of TM and generated hypoallergenic GTM and how GTM absorbed to the Al(OH)(3) function as a candidate immunotherapy for desensitizing allergy. As the results, in comparison to TM, the saccharides of smaller molecular sizes could lead to more advanced glycation end products in GTMs than saccharides of greater molecular sizes, and TM glycated by saccharides of different molecular sizes (glucose, maltose, maltotriose, maltopentaose, and maltoheptaose) exhibited lower allergenicity as a hypoallergen upon activating the allergic reactions of the mast cell and mouse model, while TM glycated by maltose had insignificant allergenicity changes upon activating the allergic reactions of the mast cell and mouse model. In addition, the hypoallergenic GTM + Al(OH)(3) was efficient as a candidate immunotherapy; this work intended to offer preclinical data to promote GTM + Al(OH)(3) as a candidate allergen-specific immunotherapy for desensitizing the allergy reactions for patients allergic to shrimp food.
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