期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 69, 期 33, 页码 9625-9631出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.1c03283
关键词
xylitol; NADPH regeneration; genetic engineering; corncob hydrolysate; CRISPR/Cas9
资金
- National Key R&D Program of China [2018YFC1604102]
- Public Projects of Zhejiang Province [LGG21B060005]
- Research on Key Research and Development Projects in Anhui Province [202004a07020020, GXXT-2019-017]
Research has shown that by modulating intracellular NADPH supply and redox environment, the efficiency of Escherichia coli cells in producing xylitol from corncob hydrolysates can be significantly improved. The NADPH-enhanced strain 2bpgi exhibited higher xylitol production and productivity in a 15 L bioreactor fermentation.
Cofactor availability is often a rate-limiting factor in the bioconversion of xylose to xylitol. The overexpression of pentose phosphate pathway genes and the deletion of Embden-Meyerhof-Parnas pathway genes can modulate the glucose metabolic flux and increase the intracellular NADPH supply, enabling Escherichia coli cells to produce xylitol from corncob hydrolysates. The effects of zwf and/or gnd overexpression and pfkA, pfkB, and/or pgi deletion on the intracellular redox environment and xylitol production were examined. The NADPH-enhanced strain 2bpgi produced 162 g/L xylitol from corncob hydrolysates after a 76 h fed-batch fermentation in a 15 L bioreactor, which was 13.3% greater than the 143 g/L xylitol produced by the IS5-d control strain. Additionally, the xylitol productivity and xylitol yield per glucose for 2bpgi were 2.13 g/L/h and 2.50 g/g, respectively. Thus, the genetic modifications in 2bpgi significantly enhanced NADPH regeneration, making 2bpgi a potentially useful strain for the industrial-scale production of xylitol from detoxified corncob hydrolysates.
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