Chemical Composition Profiling and Biological Activities of Phenolic Compounds in Eleven Red Sorghums

The profiles of soluble and insoluble phenolic compounds in 11 commercial red sorghums (B11, B12, B13, B14, J124, J127, J138, J140, J142, J152, and J158) were investigated using ultraperformance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (UPLC/QTOF-MS) analysis. A total of 48 phenolic compounds including 35 phenolic acids and their derivatives, 12 flavonoids, and 1 proanthocyanidin were identified, and 8 phenolic compounds were reported for the first time in sorghums. Four major 3-deoxyanthocyanidins were also quantified, with their soluble forms accounting for 99.75-99.87% of the total contents. Pearson's correlation analyses indicated that 3-deoxyanthocyanidins significantly contributed to the antioxidant capacities of the red sorghums and that 5-methoxy-luteolinidin showed the strongest correlation. Besides, the soluble phenolic fraction of B13 dose-dependently inhibited the proliferation of Caco-2 cells and the secretion of IL-1 beta and NO in RAW264.7 macrophages, which might be attributed to its relatively high total phenolic (TPC), flavonoid (TFC), and proanthocyanidin content (TPAC) values and radical scavenging capacities.

Automated summary

The study analyzed the profiles of soluble and insoluble phenolic compounds in 11 commercial red sorghums using UPLC/QTOF-MS, identifying a total of 48 phenolic compounds with 8 compounds discovered for the first time in sorghums. The research found that 3-deoxyanthocyanidins significantly contributed to the antioxidant capacities of red sorghums, with 5-methoxy-luteolinidin showing the strongest correlation. Additionally, the soluble phenolic fraction of B13 dose-dependently inhibited cell proliferation and inflammation in specific cell lines.