期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 69, 期 23, 页码 6690-6700出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.0c07998
关键词
site-specific glycan; intact glycopeptides; human milk whey; LC-MS/MS
资金
- National Key Research and Development Program of China [2018YFC0910302, 2020YFE0202200]
- National Natural Science Foundation of China [21775146, 22034007, 81730016]
- LiaoNing Revitalization Talents Program
- Youth Innovation Promotion Association of CAS [2018212]
- DICP, CAS [DICP I201906]
- Innovation Academy for Precision Measurement Science and Technology, CAS
The N-glycoproteomics analysis of human milk whey revealed changes in site-specific glycans without removing the attached glycans, providing insights into the role of protein N-glycosylation during infant development.
Protein N-glycosylation in human milk whey plays a substantial role in infant health during postnatal development. Changes in site-specific glycans in milk whey reflect the needs of infants under different circumstances. However, the conventional glycoproteomics analysis of milk whey cannot reveal the changes in site-specific glycans because the attached glycans are typically enzymatically removed from the glycoproteins prior to analysis. In this study, N-glycoproteomics analysis of milk whey was performed without removing the attached glycans, and 330 and 327 intact glycopeptides were identified in colostrum and mature milk whey, respectively. Label-free quantification of site-specific glycans was achieved by analyzing the identified intact glycopeptides, which revealed 9 significantly upregulated site-specific glycans on 6 glycosites and 11 significantly downregulated sitespecific glycans on 8 glycosites. Some interesting change trends in N-glycans attached to specific glycosites in human milk whey were observed. Bisecting GlcNAc was found attached to 11 glycosites on 8 glycoproteins in colostrum and mature milk. The dynamic changes in site-specific glycans revealed in this study provide insights into the role of protein N-glycosylation during infant development.
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