期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 69, 期 28, 页码 8038-8049出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.1c01822
关键词
HO1; alternative polyadenylation; adipogenesis; microRNAs
资金
- National Natural Science Foundation of China [31571274]
- Natural Science Fund of Shandong province of P. R. China [ZR2015CM025]
The study investigates the role of HO1 in preadipocyte differentiation and finds that the HO1 3'UTR short variant, produced by APA, escapes microRNA inhibition and has a stronger inhibitory effect on preadipocyte differentiation.
Appropriately increasing intramuscular fat content can help improve meat quality, so it is necessary to explore the internal molecular mechanism of preadipocyte differentiation. The role of heme oxygenase 1 (HO1) in cell oxidative stress, energy metabolism, cell proliferation, and differentiation has gradually been revealed. Here, we used 3'RACE to identify the full-length 3' untranslated region (3'UTR) of HO1 and found that a very short 3'UTR variant was produced by alternative polyadenylation (APA). HO1 with a long 3'UTR variant was identified as a direct target of miR155-5P and miR377-3P. Our experimental results verified the inhibitory effect of HO1 on preadipocyte differentiation. In addition, our research confirms that by escaping microRNA inhibitory effects, the HO1 3'UTR short variant produced by APA has a higher level of expression. Thus, the HO1 3'UTR short variant has a stronger inhibitory effect on the preadipocyte differentiation than the HO1 3'UTR long variants in 3T3-L1.
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