期刊
FOOD AND BIOPRODUCTS PROCESSING
卷 98, 期 -, 页码 79-85出版社
INST CHEMICAL ENGINEERS
DOI: 10.1016/j.fbp.2015.10.003
关键词
Simultaneous liquefaction; Saccharification and Fermentation (SLSF); Very high gravity (VHG); Rice; Potable ethanol; Distillers dried grains
资金
- Vietnamese Ministry of Education and Training [B2013.01.07DA]
- Ministry of Science and Technology [15/2014/HD-NDT]
In this work, a simultaneous liquefaction, saccharification, and fermentation (SLSF) process at very high gravity (VHG) of broken rice for potable ethanol production was developed at pilot scale. The SLSF-VHG process was performed in a unique fermenter, at 30 degrees C. Rice flour (RF) was dissolved in tap water to reach 311.5 g/l dry matter and then the mixture was simultaneousely liquefied, saccharified, and fermented. Thanks to the addition of a raw starch hydrolyzing enzyme containing a mixture of alpha-amylase and gluco-amylase (Stargen 002 at 991.8 GAU/kg RF), gluco-amylase (Amigase Mega L at 0.035% w/w), protease (Fermgen at 600 SAPU/kg RF), active dry yeast Saccharomyces cerevisiae (Red Ethanol at 3.5 x 10(7) cells/ml), KH2PO4 (4.8 mM), and urea (16.0 mM). Under these conditions, the SLSF-VHG process finished after 120 h and achieved an ethanol content of 17.6% v/v corresponding to 86.3% of the theoretical ethanol yield. We scaled up this SLSF process at very high gravity at pilot scale (25 l) and achieved an ethanol content of 17.0% v/v corresponding to a yield of 83.2% of the theoretical ethanol yield. Rice-based distillers dried grains (DDG) was produced from the whole stillage of SLSF process at very high gravity by being plate-filtered and dried. The obtained DDG had high contents of crude protein (47.5%) and fibers (15.8%). Our results suggest, the SLSF under VHG condition of broken rice as well as the recovery of protein-rich DDG could have a great potential for the ethanol and animal feeding industry in Vietnam. (C) 2015 The Institution of Chemical Engineers. Published by Elsevier B.V. All rights reserved.
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