Determination of Putrescine in Tiger Prawn Using an Amperometric Biosensor Based on Immobilization of Diamine Oxidase onto Ceria Nanospheres

An increase of putrescine content in tiger prawn can be related to the deterioration of its quality. Increase in putrescine consumption causes nausea, vomiting, diarrhea, allergy, and flushing in humans. To address this issue, a glassy carbon electrode modified with diamine oxidase on ceria nanoparticles was fabricated for rapid determination of putrescine in tiger prawn. Based on the amperometric response, calibrated models such as linear, Hill, and Michaelis-Menten functions were constructed. The best performing model was based on Hill analysis, yielding relative prediction error of 0.035, percentage recovery of 113.10, and root mean square error of cross validation of 0.083. All the three models were verified with extract of tiger prawn sample. Among the three models, only the amperometric biosensor based on Hill analysis showed acceptable recovery with low relative standard deviation in tiger prawn sample.