期刊
FOOD AND AGRICULTURAL IMMUNOLOGY
卷 28, 期 1, 页码 49-66出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/09540105.2016.1202211
关键词
Apramycin; monoclonal antibody; indirect competitive enzyme-linked immunosorbent assay; rapid strip test
资金
- National Natural Science Foundation of PRC [21471068]
- Key Programs from MOST [2012AA06A303, 2012BAD29B04]
- Natural Science Foundation of Jiangsu Province
- MOF
- MOE [BK20140003, BE2013613, BE2013611]
A specific monoclonal antibody (MAb) against apramycin (AP) was produced and used to develop an indirect competitive enzyme-linked immunosorbent assay (idcELISA) and a rapid testing strip for the detection of AP residues in foods. MAb exhibited negligible cross-reactivity with other aminoglycosides. Under optimized conditions in 0.01 M PBS, the half maximum inhibitory concentration (IC50) of MAb was 0.41 ng/ml with a limit of detection (LOD) of 0.15 ng/ml. The ELISA results were obtained within 90 min. The mean recoveries from all the spiked food samples were within the range of 79.02-105.49%, with coefficients of variation in the range of 2.21-11.4%. The strip test results obtained within 5 min had visual LODs in the range 2.5-5 mu g/kg (ng/ml) for all food samples tested. Therefore, the developed strip test represents a fast and convenient detection method of AP residues in foods.
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