期刊
FOOD AND AGRICULTURAL IMMUNOLOGY
卷 27, 期 5, 页码 689-699出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/09540105.2016.1148668
关键词
Pentachlorophenol; ELISA; immunochromatographic strip
资金
- Key Programs from MOST [2013AA065501, 2012BAK08B01]
- Natural Science Foundation of Jiangsu Province
- MOF
- MOE [BK20140003, BK20150145, BK20150138, BE2013613, BE2013611]
A sensitive monoclonal antibody for targeting pentachlorophenol was prepared with an immunization procedure and cell fusion. The antibody was characterized with an indirect competitive enzyme-linked immunosorbent assay (ELISA), which had an IC50 of 0.2ng/ml for pentachlorophenol under optimized conditions. The cross-reactivity of the ELISA to related compounds was very low. The recovery of pentachlorophenol was 99.2-105% in water samples and 89-110.9% in fish samples. A simple, rapid, and sensitive immunochromatographic strip was produced based on the monoclonal antibody, with a qualitative visual limit of detection of 2ng/ml in water samples and 10ng/ml in fish samples, indicating its potential utility in monitoring pentachlorophenol in the field.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据