期刊
出版社
MICROBIOLOGY SOC
DOI: 10.1099/ijsem.0.004960
关键词
autolysis; bile resistance; human bile isolate; Ruminococcoides
类别
资金
- MINECO (Spanish Ministry of Economy and Competitiveness) - FEDER (The European Regional Development Funds) [AGL2013-44761-P]
- FPI Predoctoral Grant [BES-2014-068736]
- 'Ramon y Cajal' program from MINECO [RYC-2016-19726]
- 'Plan for Research, Development and Innovation of the Principado de Asturias 2018-2020' - FEDER funds [IDI/2018/000236]
- Scottish Government Rural and Environmental Sciences and Analytical Services (SG-RESAS)
- Tenovus Scotland
A novel strain Ruminococcoides bili gen. nov., sp. nov. was isolated from a human bile sample, showing characteristics of starch degradation, bile tolerance, and autolysis. Phylogenetic, genomic, and metabolic analyses support its classification within the Ruminococcoides genus, with strain IPLA60002(T) proposed as the type strain.
A strictly anaerobic, resistant starch-degrading, bile-tolerant, autolytic strain, IPLA60002(T), belonging to the family Ruminococ-caceae, was isolated from a human bile sample of a liver donor without hepatobiliary disease. Cells were Gram- stain-positive cocci, and 16S rRNA gene and whole genome analyses showed that Ruminococcus bromii was the phylogenetically closest related species to the novel strain IPLA60002(T), though with average nucleotide identity values below 90 %. Biochemically, the new isolate has metabolic features similar to those described previously for gut R. bromii strains, including the ability to degrade a range of different starches. The new isolate, however, produces lactate and shows distinct resistance to the pres-ence of bile salts. Additionally, the novel bile isolate displays an autolytic phenotype after growing in different media. Strain IPLA60002(T) is phylogenetically distinct from other species within the genus Ruminococcus. Therefore, we propose on the basis of phylogenetic, genomic and metabolic data that the novel IPLA60002(T) strain isolated from human bile be given the name Ruminococcoides bili gen. nov., sp. nov., within the new proposed genus Ruminococcoides and the family Ruminococcaceae. Strain IPLA60002(T) (=DSM 110008(T)=LMG 31505(T)) is proposed as the type strain of Ruminococcoides bili.
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