期刊
出版社
MICROBIOLOGY SOC
DOI: 10.1099/ijsem.0.005005
关键词
Blautia intestinalis; faeces; human intestinal microbiota; strain 27-44T
类别
资金
- Key Research Programme of the Chinese Academy of Sciences (Strategic Priority CAS Project) [XDB38020000]
- National Nature Science Foundation of China [31670102]
- Autism Special Fund from Peking Union Medical Foundation
- CAMS Innovation Fund for Medical Science (CIFMS) [2017-I2M-3-017]
A novel strictly anaerobic bacterial strain, designated as Blautia intestinalis, was isolated from a stool specimen of an autistic child in China. The strain exhibited specific growth conditions, cell morphology, and lipid composition, and was phylogenetically closely related to other members of the genus Blautia.
A strictly anaerobic bacterial strain (27-44T) was isolated from a stool specimen from an autistic child collected in PR China. The strain was Gram- stain- positive, non- motile, non- pigmented, non- spore- forming, and cells were oval to rod- shaped. Strain 27-44T grew at 20-40 degrees C (optimal at 37 degrees C) and at pH 6.0-10 (optimal at 6.0-8.0). The major polar lipids were one phospholipid, two glycolipids, two aminophospholipids and one unidentified lipid. The major cellular fatty acids of strain 27-44T were C16: 0 and C17: 0 2- OH. The end product of glucose fermentation was mainly butyric acid. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 27-44T was a member of the genus Blautia and phylogenetically closely related to Blautia obeum ATCC 29174T (with 97.8 % seque nce similarity). The genome of strain 27-44T was 3.5 Mbp with a DNA G+C content of 42.36 mol%. A total of 3436 genes were predicted and, of these, 3133 genes were annotated by KEGG. On the basis of phenotypic, chemotaxonomic and phylogenetic comparisons, strain 27-44T represents a novel species within the genus Blautia, for which the name Blautia intestinalis sp. nov. is proposed. The type strain is 27-44T= CGMCC 1.5285T=NBRC 113774T.
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