Sediminihaliea albiluteola gen. nov., sp. nov., a new member of the family Halieaceae, isolated from marine sediment

A Gram-stain-negative, rod-shaped and facultatively aerobic bacterial strain, designated F7430(T), was isolated from coastal sediment collected at Jingzi Wharf in Weihai, PR China. Cells of strain F7430(T) were 0.3-0.4 mu m wide, 2.0-2.6 mu m long, non flagellated, non-motile and formed pale beige colonies. Growth was observed at 4-40 degrees C (optimum, 30 degrees C), pH 6.0-9.0 (optimum, pH 7.5-8.0) and at NaCl concentrations of 1.0-10.0 % (w/v; optimum, 1.0 %). The sole respiratory quinone of strain F7430(T) was ubiquinone 8 and the predominant cellular fatty acids were summed feature 8 (C-18:1 omega 7c / C-18:1 omega 6c; 60.7 %), summed feature 3 (C-16:1 omega 7c/C-16:1 omega 6c; 30.2 %) and C-15:0 iso (13.9 %). The polar lipids of strain F7430(T) consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, one unidentified phospholipid and three unidentified lipids. Results of 16S rRNA gene sequences analyses indicated that this strain belonged to the family Halieaceae and had high sequence similarities to Parahaliea aestuarii JCM 51547(T) (95.3 %) and Halioglobus pacificus DSM 27932(T) (95.2 %) followed by 92.9-95.0 % sequence similarities to other type species within the aforementioned family. The rpoB gene sequences analyses indicated that the novel strain had the highest sequence similarities to Parahaliea aestuarii JCM 51547(T) (82.2 %) and Parahaliea mediterranea DSM 21924(T) (82.2 %) followed by 75.2-80.5 % sequence similarities to other type species within this family. Phylogenetic analyses showed that strain F7430(T) constituted a monophyletic branch clearly separated from the other genera of family Halieaceae. Whole-genome sequencing of strain F7430(T) revealed a 3.3 Mbp genome size with a DNA G+C content of 52.6 mol%. The genome encoded diverse metabolic pathways including the Entner-Doudoroff pathway, assimilatory sulphate reduction and biosynthesis of dTDP-L-rhamnose. Based on results from the current polyphasic study, strain F7430(T) is proposed to represent a novel species of a new genus within the family Halieaceae, for which the name Sediminihaliea albiluteola gen. nov., sp. nov. is proposed. The type strain of the type species is F7430(T) (=KCTC 72873(T)=MCCC 1H00420T).

Automated summary

The bacterial strain F7430(T) isolated from coastal sediment in Weihai, PR China, was identified as a novel species within the family Halieaceae, named Sediminihaliea albiluteola gen. nov., sp. nov. The strain exhibited unique physiological and genetic characteristics, distinguishing it as a new species within this family.