期刊
INTERNATIONAL JOURNAL OF PHARMACEUTICS
卷 606, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.ijpharm.2021.120846
关键词
Antimicrobial; Antibiofilm; Poly(epsilon-caprolactone) nanocapsule; Essential oils; Label-free Raman imaging
资金
- VEGA Agency [2/0059/19, 2/0055/20]
This study demonstrates the effectiveness of poly(epsilon-caprolactone) nanocapsules loaded with thyme and oregano essential oils in reducing microbial and biofilm growth without cytotoxicity on human keratinocyte cells, as well as the absence of DNA damage induction.
Essential oils (EOs) of Thymus capitatus (Th) carvacrol chemotype and Origanum vulgare (Or) thymol and carvacrol chemotype were encapsulated in biocompatible poly(epsilon-caprolactone) nanocapsules (NCs). These nanosystems exhibited antibacterial, antifungal, and antibiofilm activities against Staphylococcus aureus, Escherichia coli, and Candida albicans. Th-NCs and Or-NCs were more effective against all tested strains than pure EOs and at the same time were not cytotoxic on HaCaT (T0020001) human keratinocyte cell line. The genotoxic effects of EO-NCs and EOs on HaCaT were evaluated using an alkaline comet assay for the first time, revealing that Th-NCs and Or-NCs did not induce DNA damage compared with untreated control HaCaT cells in vitro after 24 h. The cells morphological changes were assessed by label-free live cell Raman imaging. This study demonstrate the ability of poly(epsilon-caprolactone) nanocapsules loaded with thyme and oregano EOs to reduce microbial and biofilm growth and could be an ecological alternative in the development of new antimicrobial strategies.
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