期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 22, 期 12, 页码 -出版社
MDPI
DOI: 10.3390/ijms22126428
关键词
Skullcapflavone II; TARC; MDC; CTSS; STAT1; NF-kappa B; p38 MAPK; anti-inflammatory activity; atopic dermatitis; keratinocytes
资金
- National Research Foundation (NRF) - Ministry of Science ICT [2019M3A9I3091696]
- National Research Foundation of Korea [2019M3A9I3091696] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
This study found that SFII inhibits the expression of TARC, MDC, and CTSS induced by TNF-alpha/IFN-gamma by regulating the STAT1, NF-kappa B, and p38 MAPK signaling pathways.
Skullcapflavone II (SFII), a flavonoid derived from Scutellaria baicalensis, has been reported to have anti-inflammatory properties. However, its therapeutic potential for skin inflammatory diseases and its mechanism are unknown. Therefore, this study aimed to investigate the effect of SFII on TNF-alpha/IFN-gamma-induced atopic dermatitis (AD)-associated cytokines, such as thymus- and activation-regulated chemokine (TARC) and macrophage-derived chemokine (MDC). Co-stimulation with TNF-alpha/IFN-gamma in HaCaT cells is a well-established model for induction of pro-inflammatory cytokines. We treated cells with SFII prior to TNF-alpha/IFN-gamma-stimulation and confirmed that it significantly inhibited TARC and MDC expression at the mRNA and protein levels. Additionally, SFII also inhibited the expression of cathepsin S (CTSS), which is associated with itching in patients with AD. Using specific inhibitors, we demonstrated that STAT1, NF-kappa B, and p38 MAPK mediate TNF-alpha/IFN-gamma-induced TARC and MDC, as well as CTSS expression. Finally, we confirmed that SFII significantly suppressed TNF-alpha/IFN-gamma-induced phosphorylation of STAT1, NF-kappa B, and p38 MAPK. Taken together, our study indicates that SFII inhibits TNF-alpha/IFN-gamma-induced TARC, MDC, and CTSS expression by regulating STAT1, NF-kappa B, and p38 MAPK signaling pathways.
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