Complexome Profiling: Assembly and Remodeling of Protein Complexes

Many proteins have been found to operate in a complex with various biomolecules such as proteins, nucleic acids, carbohydrates, or lipids. Protein complexes can be transient, stable or dynamic and their association is controlled under variable cellular conditions. Complexome profiling is a recently developed mass spectrometry-based method that combines mild separation techniques, native gel electrophoresis, and density gradient centrifugation with quantitative mass spectrometry to generate inventories of protein assemblies within a cell or subcellular fraction. This review summarizes applications of complexome profiling with respect to assembly ranging from single subunits to large macromolecular complexes, as well as their stability, and remodeling in health and disease.

Automated summary

Complexome profiling is a mass spectrometry-based method that can provide insights into protein assembly, stability, and remodeling in health and disease. It combines various separation and sequencing techniques to generate detailed inventories of protein assemblies within cells.