期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 22, 期 13, 页码 -出版社
MDPI
DOI: 10.3390/ijms22136752
关键词
spaceflight; sounding rocket; microgravity; metabolomics; immune cells
资金
- DLR Space Administration on behalf of the Federal Ministry for Economic Affairs and Energy (BMWi) [50WB1219, 50WB1519]
This study conducted a metabolomics analysis on primary human macrophages to investigate the short-term and long-term effects of microgravity, revealing changes in amino acid concentration, protein degradation processes, and metabolic adaptations. The results suggest highly dynamic and robust metabolic changes occur in altered gravity, contributing significantly to understanding the integrative effects of gravity on human cells.
Microgravity acts on cellular systems on several levels. Cells of the immune system especially react rapidly to changes in gravity. In this study, we performed a correlative metabolomics analysis on short-term and long-term microgravity effects on primary human macrophages. We could detect an increased amino acid concentration after five minutes of altered gravity, that was inverted after 11 days of microgravity. The amino acids that reacted the most to changes in gravity were tightly clustered. The observed effects indicated protein degradation processes in microgravity. Further, glucogenic and ketogenic amino acids were further degraded to Glucose and Ketoleucine. The latter is robustly accumulated in short-term and long-term microgravity but not in hypergravity. We detected highly dynamic and also robust adaptative metabolic changes in altered gravity. Metabolomic studies could contribute significantly to the understanding of gravity-induced integrative effects in human cells.
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