期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 22, 期 15, 页码 -出版社
MDPI
DOI: 10.3390/ijms22158321
关键词
hybrid xerogels; chitosan; tricalcium phosphate; N-2 physisorption; t-plot; bioactivity; biodegradation; bone tissue engineering; osteoblasts; focal adhesions
资金
- Andalucia FEDER/ITI 2014-2020 Grant [PI 013/017]
- Junta de Andalucia PAIDI research groups (Spain) [TEP115, CTS 253]
- ERDF Operational Programme
- Department of Economy, Knowledge, Business and University of the Regional Government of Andalusia [FEDER-UCA18_106598]
Silica/biopolymer hydrogel-based materials are attractive for biomedical applications, especially for bone repair. This study introduced a synthesis method for silica-chitosan-tricalcium phosphate xerogels and investigated the effects of washing solvents and TCP content. It was found that ethanol-washed samples released both calcium and silicon ions, while water-washed samples only released silicon ions. All samples showed in vitro bioactivity and promoted cell growth.
Silica/biopolymer hydrogel-based materials constitute very attractive platforms for various emerging biomedical applications, particularly for bone repair. The incorporation of calcium phosphates in the hybrid network allows for designing implants with interesting biological properties. Here, we introduce a synthesis procedure for obtaining silica-chitosan (CS)-tricalcium phosphate (TCP) xerogels, with CS nominal content varying from 4 to 40 wt.% and 10 to 20 wt.% TCP. Samples were obtained using the sol-gel process assisted with ultrasound probe, and the influence of ethanol or water as washing solvents on surface area, micro- and mesopore volume, and average pore size were examined in order to optimize their textural properties. Three washing solutions with different soaking conditions were tested: 1 or 7 days in absolute ethanol and 30 days in distilled water, resulting in E1, E7, and W30 washing series, respectively. Soaked samples were eventually dried by evaporative drying at air ambient pressure, and the formation of interpenetrated hybrid structures was suggested by Fourier transformed infrared (FTIR) spectroscopy. In addition the impact that both washing solvent and TCP content have on the biodegradation, in vitro bioactivity and osteoconduction of xerogels were explored. It was found that calcium and phosphate-containing ethanol-washed xerogels presented in vitro release of calcium (2-12 mg/L) and silicon ions (similar to 60-75 mg/L) after one week of soaking in phosphate-buffered saline (PBS), as revealed by inductive coupled plasma (ICP) spectroscopy analysis. However, only the release of silicon was detected for water-washed samples. Besides, all the samples exhibited in vitro bioactivity in simulated body fluid (SBF), as well as enhanced in vitro cell growth and also significant focal adhesion development and maturation.
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