期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 22, 期 18, 页码 -出版社
MDPI
DOI: 10.3390/ijms22189682
关键词
Cystatin C; aggregation; cathepsin activity; Amyloid beta; neurodegeneration
The study found that Cystatin C aggregation in cerebral amyloid angiopathy may alter the protein's activity, leading to cellular degeneration and toxicity.
Cystatin C (CST3) is an endogenous cysteine protease inhibitor, which is implicated in cerebral amyloid angiopathy (CAA). In CAA, CST3 is found to be aggregated. The purpose of this study is to investigate whether this aggregation could alter the activity of the protein relevant to the molecular pathology of CAA. A system of CST3 protein aggregation was established, and the aggregated protein was characterized. The results showed that CST3 aggregated both at 80 degrees C without agitation, and at 37 degrees C with agitation in a time-dependent manner. However, the levels of aggregation were high and appeared earlier at 80 degrees C. Dot-blot immunoassay for oligomers revealed that CST3 could make oligomeric aggregates at the 37 degrees C condition. Electron microscopy showed that CST3 could make short fibrillary aggregates at 37 degrees C. Cathepsin B activity assay demonstrated that aggregated CST3 inhibited the enzyme activity less efficiently at pH 5.5. At 7.4 pH, it lost the inhibitory properties almost completely. In addition, aggregated CST3 did not inhibit A beta(1-40) fibril formation, rather, it slightly increased it. CST3 immunocytochemistry showed that the protein was positive both in monomeric and aggregated CST3-treated neuronal culture. However, His6 immunocytochemistry revealed that the internalization of exogenous recombinant CST3 by an astrocytoma cell culture was higher when the protein was aggregated compared to its monomeric form. Finally, MTT cell viability assay showed that the aggregated form of CST3 was more toxic than the monomeric form. Thus, our results suggest that aggregation may result in a loss-of-function phenotype of CST3, which is toxic and responsible for cellular degeneration.
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