期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 22, 期 12, 页码 -出版社
MDPI
DOI: 10.3390/ijms22126254
关键词
mastitis; MeRIP-seq; E; coli; m(6)A modification; N-6-methyladenosine
资金
- National Key Research and Development Plan [2016YFD0500906]
- special fund for the China Agriculture Research System (Beef/Yak cattle) [CARS-37]
- National Natural Science Foundation of China [31101874, 31972758]
- Key R&D program of Hubei province of China [2020BBA055]
- Research Fund for National Distinguished Scholars in Agriculture Research and Technical Innovative Team
This study investigated the role and mechanism of RNA m(6)A modification in mastitis treated with E. coli using MeRIP-seq technology, revealing the significant impact of m(6)A in mastitis and elucidating the interplay between gene expression and m(6)A modification, providing important insights for future research.
Mastitis is a common disease in dairy cows that is mostly caused by E. coli, and it brings massive losses to the dairy industry. N-6-Methyladenosine (m(6)A), a methylation at the N-6 position of RNA adenine, is a type of modification strongly associated with many diseases. However, the role of m(6)A in mastitis has not been investigated. In this study, we used MeRIP-seq to sequence the RNA of bovine mammary epithelial cells treated with inactivated E. coli for 24 h. In this in vitro infection model, there were 16,691 m(6)A peaks within 7066 mRNA transcripts in the Con group and 10,029 peaks within 4891 transcripts in the E. coli group. Compared with the Con group, 474 mRNAs were hypermethylated and 2101 mRNAs were hypomethylated in the E. coli group. Biological function analyses revealed differential m(6)A-modified genes mainly enriched in the MAPK, NF-kappa B, and TGF-beta signaling pathways. In order to explore the relationship between m(6)A and mRNA expression, combined MeRIP-seq and mRNA-seq analyses revealed 212 genes with concomitant changes in the mRNA expression and m(6)A modification. This study is the first to present a map of RNA m(6)A modification in mastitis treated with E. coli, providing a basis for future research.
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