期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 22, 期 17, 页码 -出版社
MDPI
DOI: 10.3390/ijms22179625
关键词
cardiomyopathy; pediatric; troponin mutation; skinned cardiomyocytes; skinned fibers; thin filaments; contractile function; levosimendan; EGCg
资金
- DFG [MA 807/19-3, HA 7512/2-1, HA 7512/2-4]
- University of Cologne (Koln Fortune)
- German Academic Exchange Service (DAAD)
- European HCEMM grant
Rare pediatric non-compaction and restrictive cardiomyopathy are usually associated with rapid and severe disease progression, with poorly understood molecular mechanisms. This study compared the molecular effects of two infantile de novo point mutations in TNNC1 and TNNI3. Although both mutations exhibited similar functional and structural impairments, patients developed different phenotypes. Protein quality control system was affected, and two troponin targeting agents showed similar effects in ameliorating contractile function.
Rare pediatric non-compaction and restrictive cardiomyopathy are usually associated with a rapid and severe disease progression. While the non-compaction phenotype is characterized by structural defects and is correlated with systolic dysfunction, the restrictive phenotype exhibits diastolic dysfunction. The molecular mechanisms are poorly understood. Target genes encode among others, the cardiac troponin subunits forming the main regulatory protein complex of the thin filament for muscle contraction. Here, we compare the molecular effects of two infantile de novo point mutations in TNNC1 (p.cTnC-G34S) and TNNI3 (p.cTnI-D127Y) leading to severe non-compaction and restrictive phenotypes, respectively. We used skinned cardiomyocytes, skinned fibers, and reconstituted thin filaments to measure the impact of the mutations on contractile function. We investigated the interaction of these troponin variants with actin and their inter-subunit interactions, as well as the structural integrity of reconstituted thin filaments. Both mutations exhibited similar functional and structural impairments, though the patients developed different phenotypes. Furthermore, the protein quality control system was affected, as shown for TnC-G34S using patient's myocardial tissue samples. The two troponin targeting agents levosimendan and green tea extract (-)-epigallocatechin-3-gallate (EGCg) stabilized the structural integrity of reconstituted thin filaments and ameliorated contractile function in vitro in some, but not all, aspects to a similar degree for both mutations.
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