期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 22, 期 13, 页码 -出版社
MDPI
DOI: 10.3390/ijms22137104
关键词
colistin resistance; FTIR spectroscopy; Raman spectroscopy; zeta-potential; Klebsiella pneumoniae
This study evaluated the electrical potential and chemical alterations in laboratory-induced colistin-resistant Klebsiella pneumoniae compared to susceptible strains, revealing differences in cell wall structures and LPS composition between the two strains.
This study evaluates the electrical potential and chemical alterations in laboratory-induced colistin-resistant Klebsiella pneumoniae, as compared to the susceptible strain using spectroscopic analyses. The minimal inhibitory concentration (MIC) of colistin, zeta-potential and chemical composition analysis of K. pneumoniae strains are determined. The results obtained for the K. pneumoniae(Col-R) with induced high-level colistin resistance (MIC = 16.0 +/- 0.0 mg/L) are compared with the K. pneumoniae(Col-S) strain susceptible to colistin (MIC = 0.25 +/- 0.0 mg/L). Fourier transform infrared (FTIR) and Raman spectroscopic studies revealed differences in bacterial cell wall structures and lipopolysaccharide (LPS) of K. pneumoniae(Col-R) and K. pneumoniae(Col-S) strains. In the beginning, we assumed that the obtained results could relate to a negative charge of the bacterial surface and different electrostatic interactions with cationic antibiotic molecules, reducing the affinity of colistin and leading to its lower penetration into K. pneumoniae(Col-R) cell. However, no significant differences in the zeta-potential between the K. pneumoniae(Col-R) and K. pneumoniae(Col-S) strains are noticed. In conclusion, this mechanism is most probably associated with recognisable changes in the chemical composition of the K. pneumoniae(Col-R) cell wall (especially in LPS) when compared to the susceptible strain.
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