期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 22, 期 13, 页码 -出版社
MDPI
DOI: 10.3390/ijms22136650
关键词
phospholipids; lysophospholipids; fatty acid synthesis; desaturation; colorectal carcinoma; EpCAM; epithelial cells; lipidomics
资金
- Ministry of Health of the Czech Republic [15-30585A, DRO-FNOL00098892]
- Czech Ministry of Education, Youth and Sports
- European Regional Development Fund [CZ.02.1.01/0.0/0.0/15_003/0000495, CZ.02.1.01/0.0/0.0/16_019/0000868, CZ.02.1.01/0.0/0.0/16_013/0001674]
- Institute of Biophysics of the Czech Academy of Sciences [RVO: 68081707]
- Ministry of Agriculture of the Czech Republic [RO0520]
The development of colon cancer is accompanied by significant lipid alterations, particularly in terms of fatty acid profiles within tumor cells. These changes are associated with increased expression of fatty acid synthesis genes and altered expression of enzymes involved in fatty acid elongation and desaturation. Additionally, specific lysophospholipids and corresponding phospholipids show significant changes in ratios, with upregulation of long-chain and very-long chain fatty acids in tumor cells.
The development of colon cancer, one of the most common malignancies, is accompanied with numerous lipid alterations. However, analyses of whole tumor samples may not always provide an accurate description of specific changes occurring directly in tumor epithelial cells. Here, we analyzed in detail the phospholipid (PL), lysophospholipid (lysoPL), and fatty acid (FA) profiles of purified EpCAM(+) cells, isolated from tumor and adjacent non-tumor tissues of colon cancer patients. We found that a number of FAs increased significantly in isolated tumor cells, which also included a number of long polyunsaturated FAs. Higher levels of FAs were associated with increased expression of FA synthesis genes, as well as with altered expression of enzymes involved in FA elongation and desaturation, including particularly fatty acid synthase, stearoyl-CoA desaturase, fatty acid desaturase 2 and ELOVL5 fatty acid elongase 5 We identified significant changes in ratios of specific lysoPLs and corresponding PLs. A number of lysophosphatidylcholine and lysophosphatidylethanolamine species, containing long-chain and very-long chain FAs, often with high numbers of double bonds, were significantly upregulated in tumor cells. Increased de novo synthesis of very long-chain FAs, or, altered uptake or incorporation of these FAs into specific lysoPLs in tumor cells, may thus contribute to reprogramming of cellular phospholipidome and membrane alterations observed in colon cancer.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据