4.7 Article

Establishment of Canine Transitional Cell Carcinoma Cell Lines Harboring BRAF V595E Mutation as a Therapeutic Target

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Article Veterinary Sciences

Longitudinal assessment of B-RAF V595E levels in the peripheral cell-free tumor DNA of a 10-year-old spayed female Korean Jindo dog with unresectable metastatic urethral transitional cell carcinoma for monitoring the treatment response to a RAF inhibitor (sorafenib)

Jung-Hyun Kim et al.

Summary: Transitional cell carcinoma (TCC) is the most common malignant tumor of the canine urinary tract. In this case study, a dog with metastatic urethral TCC was treated with sorafenib, a multi-tyrosine kinase inhibitor. The overexpression of VEGFR and presence of B-RAF V595E mutation in tumor tissue were identified. Sorafenib therapy led to alleviation of dysuria and changes in ctDNA associated with B-RAF V595E mutation were observed during the treatment period. This study suggests that ctDNA could be a useful non-invasive tool for monitoring treatment response to anticancer drugs.

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