期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 22, 期 11, 页码 -出版社
MDPI
DOI: 10.3390/ijms22116094
关键词
SARS-CoV-2 3 '-UTR; hsa-miR-3941; hsa-miR-138-5p; antiviral defense
资金
- Fondo Solidario COVID-19 of Globalcaja Foundation
- Consejeria de Educacion, Cultura y Deportes deCastilla-LaMancha government [SBPLY/17/180501/000376]
- Fundacion del Hospital Nacional de Paraplejicos para la Investigacion y la Integracion (FUHNPAIIN)
- European Union (FEDER) A way to make Europe
The study identified 10 potential candidates of human microRNAs targeting the SARS-CoV-2 genome through bioinformatics screening. Among them, hsa-miR-3941 and hsa-miR-138-5p showed effective targeting of the virus genome and complementary antiviral or protective effects in host cells.
COVID-19 pandemic is caused by betacoronavirus SARS-CoV-2. The genome of this virus is composed of a single strand of RNA with 5' and 3-UTR flanking a region of protein-coding ORFs closely resembling cells' mRNAs. MicroRNAs are endogenous post-transcriptional regulators that target mRNA to modulate protein expression and mediate cellular functions, including antiviral defense. In the present study, we carried out a bioinformatics screening to search for endogenous human microRNAs targeting the 3-UTR of SARS-CoV-2. Results from the computational techniques allowed us to identify 10 potential candidates. The capacity of 3 of them, together with hsa-miR138-5p, to target the SARS-CoV-2 3-UTR was validated in vitro by gene reporter assays. Available information indicates that two of these microRNAs, namely, hsa-miR-3941 and hsa-miR-138-5p, combine effective targeting of SARS-CoV-2 genome with complementary antiviral or protective effects in the host cells that make them potential candidates for therapeutic treatment of most, if not all, COVID-19 variants known to date. All information obtained while conducting the present analysis is available at Open Science Framework repository.
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