期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 22, 期 11, 页码 -出版社
MDPI
DOI: 10.3390/ijms22115608
关键词
carbon dots; fluorescence microspectroscopy; cnucleus; nucleolus; cytotoxicity; genotoxicity; fibroblasts; NIH; L929
资金
- Slovenian Research Agency [CZ.02.1.01/0.0/0.0/16_019/0000754, Republic-MZCR-NU21-09-00357]
- [P1-0060]
The study reveals that QCDs can penetrate the cell nucleus and exhibit concentration-dependent effects internally. QCDs have different impacts on NIH/3T3 and L929 cells, with the former experiencing changes in cell cycle profile and the latter leading to cellular death. Additionally, the presence of QCDs in the nucleus affects the intranuclear environment and causes fluorescence blue shifts.
It is important to understand the nanomaterials intracellular trafficking and distribution and investigate their targeting into the nuclear area in the living cells. In our previous study, we firstly observed penetration of nonmodified positively charged carbon dots decorated with quaternary ammonium groups (QCDs) into the nucleus of mouse NIH/3T3 fibroblasts. Thus, in this work, we focused on deeper study of QCDs distribution inside two healthy mouse NIH/3T3 and L929 cell lines by fluorescence microspectroscopy and performed a comprehensive cytotoxic and DNA damage measurements. Real-time penetration of QCDs across the plasma cell membrane was recorded, concentration dependent uptake was determined and endocytic pathways were characterized. We found out that the QCDs concentration of 200 mu g/mL is close to saturation and subsequently, NIH/3T3 had a different cell cycle profile, however, no significant changes in viability (not even in the case with QCDs in the nuclei) and DNA damage. In the case of L929, the presence of QCDs in the nucleus evoked a cellular death. Intranuclear environment of NIH/3T3 cells affected fluorescent properties of QCDs and evoked fluorescence blue shifts. Studying the intracellular interactions with CDs is essential for development of future applications such as DNA sensing, because CDs as DNA probes have not yet been developed.
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