期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 22, 期 17, 页码 -出版社
MDPI
DOI: 10.3390/ijms22179554
关键词
OsHXK1; high yield; high photosynthetic efficiency; CRISPR; Cas9; photosynthesis-related gene expression
资金
- Major Program of Guangdong Basic and Applied Research [2019B030302006]
- National Key Research and Development Program of China [2016YFD0100405]
- National Natural Science Foundation of China [32000457]
- China Postdoctoral Science Foundation [2019M662941]
- Natural Science Foundation of Guangdong Province [2019A1515110824]
The study successfully knocked out the hexokinase gene OsHXK1 in rice using the CRISPR/Cas9 gene-editing method, resulting in increased photosynthetic efficiency and yields in multiple indica rice varieties. The findings highlight the potential of gene-editing technologies in improving rice production and shed light on the roles of OsHXK1 in regulating photosynthesis and yield.
Rice (Oryza sativa L.) is an important food crop species in China. Cultivating high-yielding rice varieties that have a high photosynthetic efficiency is an important goal of rice breeding in China. In recent years, due to the continual innovation of molecular breeding methods, many excellent genes have been applied in rice breeding, which is highly important for increasing rice yields. In this paper, the hexokinase gene OsHXK1 was knocked out via the CRISPR/Cas9 gene-editing method in the indica rice varieties Huanghuazhan, Meixiangzhan, and Wushansimiao, and OsHXK1-CRISPR/Cas9 lines were obtained. According to the results of a phenotypic analysis and agronomic trait statistics, the OsHXK1-CRISPR/Cas9 plants presented increased light saturation points, stomatal conductance, light tolerance, photosynthetic products, and rice yields. Moreover, transcriptome analysis showed that the expression of photosynthesis-related genes significantly increased. Taken together, our results revealed that knocking out OsHXK1 via the CRISPR/Cas9 gene-editing method could effectively lead to the cultivation of high-photosynthetic efficiency and high-yielding rice varieties. They also revealed the important roles of OsHXK1 in the regulation of rice yield and photosynthesis.
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