期刊
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
卷 348, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.ijfoodmicro.2021.109224
关键词
Alternaria alternata; Antifungal mechanism; Cell membrane
资金
- National Key Research and Development Program [2018YFD0401302]
- China Ministry of Science and Technology
The study found that e-PL has significant inhibitory effects on black spot rot in apples, jujubes, and tomatoes, mainly by disrupting the pathogen's membrane integrity and functionality. e-PL can also induce the expression of genes related to antioxidant metabolism and pathogenesis in host fruit, enhancing antifungal activity.
e-Poly-L-lysine (e-PL) is a natural antimicrobial poly-cationic peptide widely applied as a natural preservative in the food industry, whereas its application in preventing postharvest loss of fruit was largely absent. This study investigated the antifungal activity of e-PL and determined the possible mechanisms involved. The in vivo results indicated that 500 mg L-1 exogenous e-PL treatment significantly inhibited black spot rot in apple, jujube, and tomato. The lesion diameter inhibition rate was range from 20.11% to 29.09% by 500 mg L-1 e-PL treatment. e-PL exerts antifungal activity against A. alternata in vitro, the half-inhibition concentration is 160.1 mg L-1. e-PL induced morphology and ultrastructure change on the pathogen, which resulted in the inhibition of A. alternata. This was accomplished by disturbing pathogen membrane integrity and functionality. The fluorometric assay confirmed that e-PL induced endogenous reactive oxygen species formation and accumulation in A. alternata and the elicited severe lipid peroxidation that caused membrane lesions. Further, e-PL treatment enhanced the expression of genes involved in antioxidant metabolism and pathogenesis-related responses in apple fruit. These findings illustrated that e-PL exhibits multifaceted antifungal activity by the direct effect on the pathogen as well as induce host defense responses. e-PL may be conducive as a promising alternative for Alternaria rot management.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据