期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 187, 期 -, 页码 373-385出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2021.07.150
关键词
Iron containing superoxide dismutase; Cohnella sp; Heterologous expression; Broad range of pH and temperatures
资金
- National Institute for Genetic Engineering and Biotechnology (NIGEB) , Iran
This study investigated an iron-containing type of superoxide dismutase from a novel thermophilic bacteria, showing good stability and broad catalytic activity. The enzyme could potentially have various industrial applications in cosmetic, food, and pharmaceutical industries.
Superoxide dismutases (SODs) (EC 1.15.1.1) are well known antioxidant enzymes that play critical roles in cellular defenses of living organisms against harmful superoxide radicals during oxidative stress. This study details on cloning, biochemical and functional characterization of an iron containing type superoxide dismutase (SOD) from a novel thermophilic bacteria Cohnella sp. A01 (CaSOD). The secondary and three dimensional structure of the protein were predicted. CaSOD gene was subsequently cloned into pET-26b(+) expression vector and expression of the recombinant protein (rCaSOD) was optimized in E. coli BL21 (DE3) and the purified recombinant SOD showed a single band with an apparent molecular weight of 26 kDa by SDS-PAGE. The half-life and thermodynamic parameters including Delta H*, Delta S*, and Delta G* were 187 min at 60 degrees C, 7.3 kJ.mol-1, -76.8 kJ. mol- 1.degrees K- 1, and 84.1 kJ.mol-1, respectively. The rCaSOD exhibited catalytic activity in a very broad range of pH (6.0-10.0) and temperatures (35-75 degrees C), as well as stability in a broad pH range, from 3.0 to 11.0, and wide range of temperature, different concentrations of detergent agents, metal ions, organic solvents and other chemicals. The results suggest that this novel enzyme could be used for various industrial applications in cosmetic, food, and pharmaceutical industries.
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