期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 184, 期 -, 页码 57-67出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2021.06.034
关键词
Beta galactosidase; Reduced graphene oxide; Immobilization; Hydrolysis products; Milk whey
资金
- UGC, New Delhi, India
β-Galactosidase was immobilized on modified nanosilver reduced graphene oxide (Ag@rGO) nanocomposite prepared by in vitro synthesis using same enzyme. The immobilized enzyme showed enhanced activity with an effectiveness factor (eta value) of 0.968, and retained 85% activity after repeated use. The enzyme displayed high activity even in the presence of inhibitory sugars, and effectively hydrolyzed lactose from whey. Additionally, the cytotoxicity and genotoxicity of Ag@rGO nanocomposite was assessed on human blood lymphocytes.
beta-Galactosidase was immobilized on modified nanosilver reduced graphene oxide (Ag@rGO) nanocomposite prepared by in vitro synthesis using same enzyme. The effectiveness factor, eta value of the immobilized enzyme was calculated to be 0.968, suggesting enhancement in enzyme activity after immobilization. The morphological structure of the crosslinked biopolymer was analyzed using electron microscopy and other characterization techniques. The kinetics displayed a decrease in Km value from 0.50 to 0.44 mmol L-1 while there was an increase in Vmax values from 0.031 to 0.039 limol min-1 mL-1. The immobilized enzyme retained 85% activity after its 10th repeated use. Inhibition constant (Ki) value suggests galactose to be a more potent inhibitor of the enzyme. Despite the inhibitory potential of these hydrolysis products, the immobilized enzyme preparation retained 44.2% activity in the presence of both inhibitory sugars. The as-synthesized nanobiocatalyst was found quite effective in hydrolyzing 89% of lactose from whey. Hence, this nanobiocatalyst can be used in removing lactose from dairy waste, whey before releasing it into the water bodies. Also, the cytotoxicity and genotoxicity of Ag@rGO NC was assessed on human blood lymphocytes using flow cytometry and comet assay, respectively.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据