期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 185, 期 -, 页码 306-316出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2021.06.114
关键词
Chimonanthus nitens Oliv; Polysaccharides; In vitro digestion; Antioxidant activity
资金
- National Natural Science Foundation of China [31560459]
- Major Discipline Academic and Technical Leaders Training Program of Jiangxi Province [20182BCB22003]
- Graduate Innovative Spe-cial Fund Projects of Jiangxi Province, China [YC2020S233]
The study revealed that COP1, derived from Chimonanthus nitens Oliv, exhibited improved radical scavenging activities through gastrointestinal digestion, prevented hepatotoxicity and oxidative damage induced by CP in mice, and regulated antioxidant enzyme activity and signaling pathways to protect against oxidative stress.
The study was aimed to investigate the simulated digestion behavior of the bioactive polysaccharides from Chimonanthus nitens Oliv (COP1), antioxidant activity in vitro, and prevention against cyclophosphamide (CP) induced oxidative damage in mice. The results showed that COP1 were 18.843 kDa, and consisted of arabinose (56.6 mol%), galactose (24.9 mol%), xylose (11.1 mol%), and glucose (7.4 mol%). Gastrointestinal digestion significantly improved the radical (DPPH center dot, center dot OH, and ABTS+) scavenging activities of COP1. Meanwhile, administration of COP1 (150, 300, and 600 mg/kg, continuous 16 days) prevented hepatotoxicity in CP-induced mice (reducing liver index and transaminase levels, alleviating liver damage). COP1 also attenuated oxidative stress as evident from as shown by reduced levels of MDA and enhanced activity of antioxidant enzymes (CAT, SOD, and GSH-Px). In addition, COP1 regulated the Nrf2/Keap1 signaling pathway in CP-treated mice, decreasing the upstream factor Keap1 and increasing the upstream factor Nrf2, which in turn enhanced the expression of downstream factors (NQO1, HO-1, GSH-Px, SOD1, and CAT). COP1 also protected the body from CP-induced oxidative damage by down-regulating Bax and caspase3 in the apoptosis pathway and up-regulating Bcl-2 mRNA levels. Overall, COP1 might be harnessed as an effective natural antioxidant for medical and food industries.
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