期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 185, 期 -, 页码 165-175出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2021.06.023
关键词
Anthracene-modified chitosan; Dual-crosslinked hydrogel; Free radical polymerization; Photo-dimerization; Cell-biomaterial interface; Stiffness-modulated cell behavior
资金
- Koc University [SF.0074]
- Scientific and Technological Research Council of Turkey (TUBITAK) under International Support Program (COST Action-European Coop-eration in Science and Technology) [116 M995, CA15216]
- Presidency of Turkey, Presidency of Strategy and Budget
This study synthesized single and dual-crosslinked anthracene-functional chitosan-based hydrogels without toxic initiators, demonstrating that the dual-crosslinked hydrogels had higher Young's modulus and cell volume compared to the single-crosslinked hydrogels, without affecting cytotoxicity. Additionally, altering the hydrogel properties can modulate cell morphology.
In this study, we report the synthesis of single and dual-crosslinked anthracene-functional chitosan-based hydrogels in the absence of toxic initiators. Single crosslinking was achieved through dimerization of anthracene, whereas dual-crosslinked hydrogel was formed through dimerization of anthracene and free radical photopolymerization of methacrylated-chitosan in the presence of non-toxic initiator riboflavin, a well-known vitamin B2. Both single and dual-crosslinked hydrogels were found to be elastic, as was determined through rheological analysis. We observed that the dual-crosslinked hydrogels exhibited higher Young's modulus than the single-crosslinked hydrogels, where the modulus for single and dual-crosslinked hydrogels were measured as 9.2 +/- 1.0 kPa and 26 +/- 2.8 kPa, respectively resulting in significantly high volume of cells in dual-crosslinked hydrogel (2.2 x 107 mu m3) compared to single-crosslinked (4.9 x 106 mu m3). Furthermore, we investigated the cytotoxicity of both hydrogels towards 3T3-J2 fibroblast cells through CellTiter-Glo assay. Finally, immunofluorescence staining was carried out to evaluate the impact of hydrogel modulus on cell morphology. This study comprehensively presents functionalization of chitosan with anthracene, uses nontoxic initiator riboflavin, modulates the degree of crosslinking through dimerization of anthracene and free radical photopolymerization, and further modulates cell behavior through the alterations of hydrogel properties.
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