期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 187, 期 -, 页码 361-372出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2021.07.118
关键词
Agglutination; Antibacterial activity; Galectin; Innate immunity; Site-directed mutagenesis; Nibea albiflora
资金
- National Key Research and Development Program of China [2018YFD0900301-09]
- National Natural Science Foundation of China [32072969, U1705231, 21706222, 31872562]
- Science and Technology Project of Fujian Province [2019J01693, 2018N5010, 2018J01450]
- New Century Excellent Talents in Fujian Province University to Hanfang [B18223]
- Ningbo Natural Science Foundation [202003N4050]
- Open Research Fund of Key Laboratory for Organic Electronics and Information Displays
- Joint Research Funds of Department of Science & Technology of Shaanxi Province
- Northwestern Polytechnical University [2020GXLH-Z-021]
- Fundamental Research Funds for the Central Universities
Galectins, including YdGal-3, play a crucial role in immune responses during pathogenic infections. The study found that YdGal-3 expression increased significantly in liver, spleen, and head kidney after infection with Vibrio harveyi. Additionally, YdGal-3 protein showed agglutinating and antibacterial activity against several gram-negative bacteria, indicating its importance in the innate immunity of Nibea albiflora.
Galectins are protein that participates in a variety of immune responses in the process of pathogenic infections. In the present study, a chimera galectin gene was screened from the transcriptome database of Nibea albiflora, which was named as YdGal-3. The results of qRT-PCR showed that the mRNA transcripts of YdGal-3 were ubiquitously distributed in all the detected tissues. After infection with Vibrio harveyi, the expression of YdGal-3 in liver, spleen, and head kidney increased significantly. Immunohistochemistry showed that YdGal-3 protein was widely expressed in the head kidney. The purified YdGal-3 protein by prokaryotic expression agglutinated red blood cells. Sugar inhibition assay showed that the agglutinating activity of YdGal-3 protein was inhibited by different sugars including lactose, D-galactose, and lipopolysaccharide. In addition, we mutated YdGal-3 His 294 into proline (P), alanine (A), glycine (G), and aspartic acid (D), it was further proved that the residue plays a key role in agglutination. YdGal-3 agglutinated some gram-negative bacteria including Pseudomonas plecoglossicida, Vibrio parahemolyticus, V. harveyi, and Aeromonas hydrophila, and exhibited antibacterial activity. These results suggested that YdGal-3 protein played an important role in the innate immunity of N. albiflora.
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