期刊
INSECT SCIENCE
卷 29, 期 3, 页码 669-682出版社
WILEY
DOI: 10.1111/1744-7917.12953
关键词
nanocarrier; RNA interference; Ultrabithorax; vestigial; wing development
类别
资金
- National Natural Science Foundation of China [32030012, 31900363]
- Beijing Natural Science Foundation [6204043]
- National Key Research and Development Program of China [2018YFD0200804]
The study successfully cloned two genes related to wing development in Myzus persicae and achieved downregulation of their expression levels through topical and root application of double-stranded RNAs, which also affected wing development, providing a promising method for controlling aphid migration.
RNA interference (RNAi) has developed rapidly as a potential green pest management strategy. At present, most studies have focused on the screening of aphid lethal genes, whereas only a few studies have been conducted on wing development, which is crucial for aphid migration and plant-virus dissemination. Here, the Myzus persicae genes vestigial (vg) and Ultrabithorax (Ubx) related to wing development, were cloned. These two genes were expressed in various tissues of 3rd-instar winged aphids. The mRNA level of vg was high in 3rd-instar nymphs, whereas the expression level of Ubx was high in adults. The nanocarrier-mediated delivery system delivered double-stranded RNAs for aphid RNAi using topical and root applications. The expression levels of vg and Ubx were downregulated by 44.0% and 36.5%, respectively, using the topical application. The simultaneous RNAi of the two target genes caused 63.3% and 32.2% wing aberration rates using topical and root applications, respectively. The current study provided a promising method for controlling aphid migration to alleviate the spread of insect transmitted plant diseases.
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