期刊
INSECT SCIENCE
卷 29, 期 2, 页码 379-387出版社
WILEY
DOI: 10.1111/1744-7917.12946
关键词
CRISPR; Cas9; development; ecdysone; silkworm; U1 snRNP
类别
资金
- National Science Foundation of China [31830093, 32021001, 31802005]
- Strategic Priority Research Program of Chinese Academy of Sciences [XDB11010600]
The U1 snRNP is highly conserved and plays a crucial role in silkworm development, as demonstrated by delayed growth and abnormal cellular phenotypes in U1 snRNP deletion mutants. RNA-seq analysis revealed significant impacts on genes related to metabolic pathways and hormone synthesis due to U1 snRNP depletion.
U1 small nuclear ribonucleoproteins (U1 snRNP) associates with 5MODIFIER LETTER PRIME splice sites in the form of ribonucleoprotein particles and is highly conserved among species. The physiological functions of U1 snRNP in a lepidopteran model insect Bombyx mori is mostly unknown. Here, we showed that U1 snRNP plays an important role in the development of silkworm. Knockout of U1 snRNP in silkworm showed either delayed or stationary 1st instar larva development compared with the wild-type group. U1 snRNP deletion mutants exhibited abnormal cellular phenotypes with enlarged cell nucleus, scant cytoplasm and enlarged nuclei. RNA-seq analysis revealed that genes involved in metabolic pathway, biosynthesis of secondary metabolites and steroid hormone biosynthesis were significantly affected by U1 snRNP depletion. Taken together, our study suggests that U1 snRNP homeostasis plays an important role in silkworm development.
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