4.6 Article

Bombyx mori U-shaped regulates the melanization cascade and immune response via binding with the Lozenge protein

期刊

INSECT SCIENCE
卷 29, 期 3, 页码 704-716

出版社

WILEY
DOI: 10.1111/1744-7917.12959

关键词

Bombyx mori; Lozenge; melanization response; silkworm; U-shaped

资金

  1. National Natural Science Foundation of China [31802142]
  2. Doctorial Start-up Fund of Southwest University [SWU120019, 020023]
  3. Fundamental Research Funds for the Central Universities [XDJK2019C089]
  4. China Postdoctoral Science Foundation [2019T120801, 2017M620408]
  5. Opening Foundation of State Key Laboratory of Silkworm Genome Biology [SKLSGB-ORP202003]

向作者/读者索取更多资源

The zinc finger protein U-shaped plays a crucial role in regulating melanization process and innate immune responses in silkworm by interacting with the Lozenge protein, especially with increased expression levels in hemocytes and upregulation after bacterial challenges.
Zinc finger protein, an important transcription factor, regulates gene expression associated with various physiological and pathological processes. U-shaped, belong to the Friend of GATA (FOG) transcription factor, plays a crucial role in hematopoiesis by interacting with the GATA transcription factor as a co-factor. However, little is known about its functions in insects. In the present study, a U-shaped cDNA was identified and characterized from the silkworm Bombyx mori and its potential roles in innate immunity investigated. The predicted silkworm U-shaped amino acid sequence contained a classical nuclear localization signal (NLS) motif GESSPKRRRR at position 450-459, and arginine residues at position 456 and 478 are the critical sites of the NLS. U-shaped mRNA was detected in all tested tissues of the B. mori; however, the highest levels were found in the hemocytes. U-shaped mRNA expression levels were upregulated in the hemocyte after the Escherichia coli and Staphylococcus aureus challenge. Furthermore, U-shaped knockdown significantly reduced the melanization process and suppressed the expression of melanization-associated genes, including PPO1, PPO2, PPAE and BAEE. In addition, U-shaped interacts with Lozenge protein to regulate the innate immune response of the insect. Our results revealed that U-shaped binds directly to Lozenge protein to modulate the melanization process and innate immune responses in silkworm.

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