期刊
INSECT SCIENCE
卷 29, 期 2, 页码 388-398出版社
WILEY
DOI: 10.1111/1744-7917.12938
关键词
Dusky; 4E-BP; Tribolium castaneum; 20-hydroxyecdysone; wing
类别
资金
- National Natural Science Foundation of China [32070501, 31601893]
- General Project of the Natural Science Foundation of the Jiangsu Higher Education Institutions [16KJB180004]
- Postgraduate Research & Practice Innovation Program of Jiangsu Province [KYCX19_1576]
- Young Talents Training Program of Jiangsu University
The zona pellucida domain protein Dusky (Dy) has been found to play a significant role in wing morphogenesis in insects by regulating cell size, metabolism, and gene expression. Dy controls 20-hydroxyecdysone and amino acid metabolism to ultimately impact wing growth and development in insects.
The zona pellucida domain protein Dusky (Dy) plays a vital role in wing morphogenesis in insects, but little information on its function has been reported. In this study, we found that dy regulated wing cell size, larval and pupal duration, and the metabolism of amino acid and 20-hydroxyecdysone in Tribolium castaneum. Using RNA-seq, 413 differentially expressed genes were identified between physiological buffer-injected and dy-double-stranded RNA-treated larvae, including 88 downregulated genes and 325 upregulated genes. Among these genes, dy knockdown increased CYP18A1 expression to elevate the 26-hydroxylation of 20-hydroxyecdysone, which ultimately led to growth defects in wing cells. Silencing of dy upregulated the transcription of genes encoding tyrosine aminotransferase, 4-hydroxyphenylpyruvate dioxygenase, homogentisate 1, 2-dioxygenase, and Pale to promote the catabolism of tyrosine and phenylalanine, which eventually reduced amino acid content. Furthermore, dy knockdown upregulated 4E-BP expression, and 4E-BP silencing partially phenocopied dy RNA interference-mediated wing morphogenesis. These results suggest that Dy controls 20-hydroxyecdysone and amino acid metabolism to regulate wing morphogenesis in the insect.
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